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Hold

Spanish translation: mantener / manteniendo / esperar / esperando

10:06 May 9, 2021
English to Spanish translations [PRO]
COVID-19 - Medical - Genetics
English term or phrase: Hold
These are the instructions for pcr extraction kit.
It saysl like this:
Run the PCR protocol.
Stage Temperature,°C Time Number of cycles
Hold 50 15 min 1
Hold 94 3 min 1
Cycling 94 10 s 5

My question is: What does "hold" mean on this context? what possible translation in Spanish?
Thanks a lot
Rosario Sanz
United Kingdom
Local time: 12:42
Spanish translation:mantener / manteniendo / esperar / esperando
Explanation:
En esa columna de la tabla, "hold" es un nombre indicativo de esa etapa o paso, donde simplemente se debe mantener el material a cierta temperatura durante cierto tiempo para que se produzca determinado avance de reacciones. Por lo tanto se está "manteniendo" a temperatura, o se está "esperando" que pase cierto lapso de tiempo.

Una referencia discutida en Kudoz ("hold for 10 minutes") en contexto de procedimiento químico está en:
https://www.proz.com/kudoz/english-to-spanish/chemistry-chem...
Se respondió "mantenga durante ..."
La pregunta actual se refiere exactamente a lo mismo.

Referencias para "hold" en PCR:

"PCR Process
Basic PCR can be split into three general stages: denaturation, annealing and extension. Typically, a PCR protocol consists of an initial denaturation step, around 30 cycles of these three stages, a final extension step, and a holding step with a temperature of 4-10°C. Shown below is one PCR cycle detailing the different stages and the corresponding temperatures. "
https://www.goldbio.com/goldbios-pcr-overview

"The cycling is often preceded by a single temperature step at a very high temperature (>90 °C (194 °F)), and followed by one hold at the end for final product extension or brief storage. The temperatures used and the length of time they are applied in each cycle depend on a variety of parameters,"
..."Final hold: The final step cools the reaction chamber to 4–15 °C (39–59 °F) for an indefinite time, and may be employed for short-term storage of the PCR products."
https://en.wikipedia.org/wiki/Polymerase_chain_reaction
Selected response from:

Daniel Delgado
Argentina
Local time: 08:42
Grading comment
4 KudoZ points were awarded for this answer



Summary of answers provided
4 +3mantener / manteniendo / esperar / esperando
Daniel Delgado


  

Answers


1 hr   confidence: Answerer confidence 4/5Answerer confidence 4/5 peer agreement (net): +3
mantener / manteniendo / esperar / esperando


Explanation:
En esa columna de la tabla, "hold" es un nombre indicativo de esa etapa o paso, donde simplemente se debe mantener el material a cierta temperatura durante cierto tiempo para que se produzca determinado avance de reacciones. Por lo tanto se está "manteniendo" a temperatura, o se está "esperando" que pase cierto lapso de tiempo.

Una referencia discutida en Kudoz ("hold for 10 minutes") en contexto de procedimiento químico está en:
https://www.proz.com/kudoz/english-to-spanish/chemistry-chem...
Se respondió "mantenga durante ..."
La pregunta actual se refiere exactamente a lo mismo.

Referencias para "hold" en PCR:

"PCR Process
Basic PCR can be split into three general stages: denaturation, annealing and extension. Typically, a PCR protocol consists of an initial denaturation step, around 30 cycles of these three stages, a final extension step, and a holding step with a temperature of 4-10°C. Shown below is one PCR cycle detailing the different stages and the corresponding temperatures. "
https://www.goldbio.com/goldbios-pcr-overview

"The cycling is often preceded by a single temperature step at a very high temperature (>90 °C (194 °F)), and followed by one hold at the end for final product extension or brief storage. The temperatures used and the length of time they are applied in each cycle depend on a variety of parameters,"
..."Final hold: The final step cools the reaction chamber to 4–15 °C (39–59 °F) for an indefinite time, and may be employed for short-term storage of the PCR products."
https://en.wikipedia.org/wiki/Polymerase_chain_reaction


Daniel Delgado
Argentina
Local time: 08:42
Native speaker of: Spanish
PRO pts in category: 12

Peer comments on this answer (and responses from the answerer)
agree  SandraV: Esperar. Saludos.
4 hrs
agree  Chema Nieto Castañón: Pues yo diría mantener; mantener (durante) un ciclo de 15 mins a 50°C
10 hrs
agree  Ruth Matosian
13 hrs
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