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Glossary entry

English term or phrase:

PEG Filtrate 0 hr Potency

Spanish translation:

filtrado de PEG, 0 hora potencía

Added to glossary by Sandra Lang
Sep 24, 2013 15:08
10 yrs ago
4 viewers *
English term

PEG Filtrate 0 hr Potency

English to Spanish Science Chemistry; Chem Sci/Eng
La traducción propuesta es: Potencia del filtrado de PEG a 0 h. Sin embargo, no estoy totalmente segura.

Esto aparece en un cuadro donde hay otras entradas con el siguiente texto:

Potency 0 hr (mg/mL
Potency 5 hr (mg/mL)a

a The 5 hr Potency value was adjusted for the volume of TNBP/Polysorbate that was not present in the 0 hr sample
Proposed translations (Spanish)
4 filtrado de PEG, 0 hora potencía

Discussion

Neil Ashby Oct 1, 2013:
@ Abe PLEASE READ THE LAST ADDITION TO MY EXPLANATION to understand how PEG is used to trap and then remove viruses. It gives a very clear definition of its use, please actually read it!
Neil Ashby Oct 1, 2013:
@ abe I was using concentration and storage of viruses as an example of usage, I also mention that the technique is used for proteins, INHIBITORS (as is the case in your reference).....etc.
Have you read all of the sources I've referenced? I read pages 4 - 6 of the source you mention. My answer is correct (the client said so ;) ) although the explanation may not be 100%. I've been trying to explain that PEG is used in various procedures for different reasons and have given references about them in my explanation. In the case of your ref. it is used in the production process for the removal/inactivation of enveloped viruses "It was convincingly demonstrated that the production process of Prolastin HS contians *****two effective steps for inactivation/removal of enveloped viruses**** (i.e. pasteurisation and the 11.5% *****PEG-Precipitation*******/depth filtration)" i.e. they use PEG to remove 'enveloped viruses' - if the PEG is solid after the precipitation and it is "removing the enveloped virus" where do you think the 'enveloped virus' is???? Trapped in the solid matrix of the PEG precipitate. The CELLULAR MEMBRANE FILTERS OFF THE PEG PPT. CONTAINING THE VIRUS.
abe(L)solano Sep 30, 2013:
@Neil, and with due respect... I´m not gonna enter into "Filtrado DE PEG-Filtrado CON PEG" discussion (since the client has the final word and it has already been said). I do not think your answer is right since you´re stating "The PEG filtrate is used to concentrate and store viruses"; then my opinion is you have a different procedure in mind to what Filgueira has described. And finally, I don´t think you have actually read the document (which I find strikingly similar to Sandra´s) since at any moment they are talking about PEG-enveloped viruses, they are talking about the removal/inactivation of viruses enveloped or not, by a cellular membrane (due to the "budding off" process while hatching from infected cells). Cheers!
Neil Ashby Sep 30, 2013:
Hi Abe Sorry but when you say MC Filgueira's line of thought is correct you are actually referring to my answer, because MC who initially said "filtrado de PEG" WAS INCORRECT (the client has since said it is correct), she also doubted the procedure (which is described briefly in my explanation).
What everyone seems to be missing is that there are various uses of PEG, one is as a molecular sieve (where you state that it is clear they are filtering, not filtrating, with PEG - however your source states that PEG is used to envelop the virus so that it is safe for handling - as per my explanation!!!).
abe(L)solano Sep 30, 2013:
more information FOR EVERYBODY... I think MC Filgueira´s line of thought is right, and then there is this procedure which I think if it´s not the same as the one Sandra is referring to, it must be something quite similar (pages 4 to 6): http://portal.dimdi.de/amispb/doc/pei/Web/2112945-paren-2006... Now, it is clear that ther are filtrating WITH PEG but why they´re calling it "Filtrado DE PEG" must be the way the "client" or manufacturer is identifying the product at certain stage through the procedure.
Neil Ashby Sep 29, 2013:
Hola MC Aún no te parece correcto hablar de "filtrado de PEG"?? ;@)
Efectivamente el filtrado contiene PEG, tal y como he explicado al principio de mi explanación.

PD la ST = the source text
Sandra Lang (asker) Sep 25, 2013:
PEG filtrate has been translated by the client as: filtrado de PEG

More context (pieces which I think may help). This is a very long an complex document with many figures.
After centrifugation, the effluent is filtered and becomes the 11.5% PEG filtrate.

Percent recovery calculated by Alpha1-PI potency of the PEG Filtrate solution divided by the potency of the Fraction IV-1 suspension solution.
M. C. Filgueira Sep 25, 2013:
¿Qué es la ST? Las suposiciones que hago, Neil, se refieren a las etapas de purificación habituales de los procesos de fabricación de biofármacos. Por eso pienso que los 'virus validation studies' son, en realidad, 'virus inactivation and removal validation studies', como en este caso:

http://www.ema.europa.eu/docs/en_GB/document_library/Scienti...

Todo puede esclarcerse completamente si Sara proporciona más contexto.
Neil Ashby Sep 25, 2013:
@ MC I've seen examples where the precipitation with PEG resulted in 'pellets' (the word used, see http://www.biocat.com/bc/pdf/LV810A-1_LV825A-1_Manual_PEGit_... "Transfer supernatant to a sterile vessel and add 1 volume of cold
PEG-it Virus Precipitation Solution at 4ºC to every 4 volumes of
Lentivector-containing supernatant. The PEG-it Virus
Precipitation Solution is a 5× solution. (Example: 5ml PEG-it with
20ml viral supernatant).").
This ref gives the term PEG-it Virus Precipitation Solution = solución de filtrado de PEG.
A mi tambíen resulta curioso pero así se dicen en inglés, tal y como es en la ST, es que la ST es incorrecto?
Saludos.
M. C. Filgueira Sep 25, 2013:
San Contexto Según el (poco) contexto proporcionado, no se trata de concentrar y purificar virus, sino de inactivarlos e eliminarlos (de la preparación de IPα1): se mencionan, además del PEG, tratamientos con TNBP y PS, así como 'virus validation studies' (supongo que se trata de 'virus inactivation and removal validation studies').

De ser así, y si la secuencia de etapas que se me ocurrió es la correcta (precipitación con PEG, filtración, inactivación de virus por tratamiento con TNBP y PS), no me parece correcto hablar de "filtrado de PEG", puesto que este sintagma parece indicar que el filtrado en cuestión contiene PEG (cuando, si mis suposiciones son correctas, debe contener relativamente poco PEG residual).
Neil Ashby Sep 25, 2013:
@ MC Gracias para confirmar (lo del uso de PEG) que he explicado brevemente en mi respuesta ;@).
Pero ojo, mientras investigaba, encontre casos claros de un distincto uso de PEG para concentrar/purificar virus, proteinas, etc., donde un 'molecular sieve' estaba impregnada con PEG para modificar el taman~o de substancias pasando atraves del mismo. Se utilizan un ultracentrifugadora para 'forza' la substancia atraves del 'molecular sieve' / PEG filter, dejando las moleculas grandes en la supernatante y pasando las impurezas al 'filtrado'
M. C. Filgueira Sep 24, 2013:
No creo que se trate de un complejo. (II) El tratamiento con tri-n-butilfosfato (TNBP) y polisorbato (PS) debe ser, precisamente, la etapa de inactivación de virus que validaron. Para ello, hay que demostrar, además de la inactivación del virus añadido, que el tratamiento con TNBP y PS no disminuye de manera significativa la potencia inhibitoria (o actividad) de la preparación de IPα1. La determinación "0 hr" [sic] corresponde a la del testigo (control), es decir, el filtrado que no fue tratado con la mezcla de TNBP y PS, y la determinación "5 hr' [sic] a la de la solución incubada durante 5 h con TNBP y PS. (Desde luego, las muestras tienen que haberse mezclado previamente con una preparación de un virus determinado).

Al combinar todo esto yo traduciría 'PEG Filtrate 0 hr Potency' como "Potencia del filtrado posterior a la precipitación con PEG, 0 h".

Si este sintagma apareciera en un cuadro, con la limitación de espacio que esto supone, utilizaría alguna abreviatura (por ejemplo, simplemente "filtrado PEG") y la aclararía en el pie, mediante una llamada.

Pero claro, para estar segura de esta traducción tendrías que confirmarme que las suposiciones que he hecho son correctas.
M. C. Filgueira Sep 24, 2013:
No creo que se trate de un complejo. (I) En mi opinión, el poli(etilenglicol) (PEG) se utilizó como precipitante en la etapa de purificación del inhibidor de la proteinasa α1 (IPα1, o α1-PI en inglés). Véase, por ejemplo:

http://www.safcglobal.com/etc/medialib/docs/SAFC/Bulletin/t0...
http://www.jbc.org/content/256/23/12108.full.pdf
http://docencia.izt.uam.mx/sgpe/files/users/uami/sho/Precipi...

Después de la etapa de precipitación con PEG hay probablemente una etapa de filtración en la que se obtiene el filtrado en cuestión. Si esto es así, yo traduciría 'PEG filtrate' como "filtrado posterior a la precipitación con PEG" o algo por el estilo.

[Sigo en otra ventana.]
abe(L)solano Sep 24, 2013:
Es un complejo PEG-PI entonces y sí, sería la potencia biológica basada en la concentración o mg/ml del compuesto empleados en la prueba.
Sandra Lang (asker) Sep 24, 2013:
Actually they are filtrating with PEG.

This is the title of the chart I mentioned originally in my question:

Analytical Results for 11.5% PEG Filtrate Alpha1-PI Potency Used in the Virus Validation Studies

Then it follows a chart with:

Sample
Experiment No. Potency 0 hr (mg/mL) Potency 5 hr (mg/mL)a Recovery (%)
abe(L)solano Sep 24, 2013:
But wait, here you´re talking about XXX potency, and then in your question is PEG they are filtrating? es en la misma parte del texto o son dos partes diferentes??
abe(L)solano Sep 24, 2013:
Then, they´re talking about Biological potency, "potencia biológica", I´m not sure my answer fits now!
Sandra Lang (asker) Sep 24, 2013:
I am not sure about "potencia" because "concentration" is also mentioned throughout the text.

I hope this information helps:

XXX potency was assayed by measuring the degree of inhibition of elastase activity which in turn is quantified by the rate of hydrolysis of a chromogenic substrate. Samples and controls are preincubated with porcine pancreatic elastase to enable the binding between elastase and XXX.
Neil Ashby Sep 24, 2013:
Hi Sandra I too would have reservations with "potencía" but can you tell us a bit more about the 'potency'? Against what is the PEF filtrate more or less 'potent' with time? It would be useful to know what happens during this period (tests, additions, physical changes i.e. temp. etc.). How is the potency analysed (inhibition of another reaction, ???)?
TIA
FlyHi Sep 24, 2013:
@Sandra, the suggested translation seems correct to me. Is there any particular reason you don't like it?

Proposed translations

2 hrs
Selected

filtrado de PEG, 0 hora potencía

PEG is added to a solution containing the virus, it is then precipitated and the virus is contained within the suspended PEG lattice (PEG is a polymer that forms a net around the virus, proteins, inhibitor, etc. - the subject of interest).
This is then filtered, leaving a "PEG filtrate" (containing the study subject). The PEG filtrate is used to concentrate and store viruses.

Inhibitors ability to nullify viruses are often assessed by their specificity and potency; the equivalent terms are used in Spanish.
especificidad y potencia

--------------------------------------------------
Note added at 3 hrs (2013-09-24 18:09:05 GMT)
--------------------------------------------------

However, although I have found papers that use PEG to 'trap' and suspend the subject, others use it as a type of molecular sieve (more to purify and concentrate the subject rather than 'trap' it).

Either way "potency" here is correct as "potencía":

Inhibidor enzimático - Wikipedia, la enciclopedia libre
es.wikipedia.org/wiki/Inhibidor_enzimático‎
La validez de un inhibidor enzimático medicinal suele venir determinada por su especificidad (su incapacidad de unirse a otras proteínas) y su potencia (su ...

Un inhibidor dual lucha contra el Párkinson de dos formas distintas ...
estaestuobra.es/.../un-inhibidor-dual-lucha-contra-el-p...‎
25 Jun 2013 - El siguiente paso consiste en optimizar este inhibidor en cuanto a potencia, especificidad y bio disponibilidad, de forma que pueda ser ...

[PPT]
Sin título de diapositiva
www.usal.es/~dbbm/clasmed/enz05.ppt‎
covalentemente a la enzima, inactivándola. - Tienen por tanto (a) la especificidad del inhibidor competitivo. y (b) la potencia de los inhibidores irreversibles.

[PDF]
Lección 2 FARMACODINAMIA ACCIÓN FARMACOLÓGICA
ocw.uv.es/ciencias.../leccion2.farmacodinamia.pdf‎
Inhibidores reversibles o irreversibles (efecto de ... La especificidad es recíproca … .... Potencia. • Concentración de fármaco necesaria para obtener un.


Inhibidor Enzimático - Documentos - Ninabelen - BuenasTareas.com
www.buenastareas.com › Página principal › Ciencia‎
Rating: 4.5 - ‎1 review
La validez de un inhibidor enzimático medicinal suele venir determinada por su especificidad (su carencia de unirse a otras proteínas) y su potencia (su ...


--------------------------------------------------
Note added at 5 days (2013-09-30 14:43:38 GMT)
--------------------------------------------------

Thanks to Abe for providing this ref. which supports my suggestion that the virus is suspended in a PEG polymer lattice.....

"It was convincingly demonstrated that the production process of Prolastin HS ***************contains two effective steps for elimination/removal of enveloped viruses (i.e. pasteurisation and the 11.5% PEG-Precipitation/depth filtration)*****************. Non-enveloped viruses are effectively removed during the PEG-Precipitation/depth filtration step."
Taken from page 6 of http://portal.dimdi.de/amispb/doc/pei/Web/2112945-paren-2006...

and on page 5 of the same document:
"Finished product testing includes tests for process-related impurities (albumin and globulins), for ************components added during manufacture (sucrose, PEG,****************** TRIS) and for excipients sodium chloride and sodium monophosphate."

THIS SHOWS THAT PEG IS PRECIPITATED THEN FILTERED GIVING A SOLID PHASE THAT ENVELOPES THE VIRUS, FOR STORAGE, INACTIVITY, ETC. AS PER THE BEGINNING OF MY EXPLANATION. THIS IS CALLED A PEG PELLET ALTHOUGH IT EFFECTIVELY CONTAINS NO PEG, SEE REF. (sorry for shouting, but nobody seems to be taken any notice of what I'm writing).

"How does PEG work in the process of purifying DNA?

I'd like to expand some of the points found in the thread
that developed in response to this question.

1) PEG forms large random coils that include water but exclude
DNA. The DNA eventually precipitates from lack of solvation.
MW of the DNA, MW of the PEG, and salt conc. all matter.
You can do crude size cuts by sequential centrifugation
with increasing concentrations of PEG.

2) Other macromolecules also precipitate, each at their characteristic
PEG concentration.

3) Though some PEG gets caught up in the pellet, most of the PEG is
left behind in the supernatent. So the "PEG pellet" is really
not a pellet of PEG.

4) PEG that carries over generally does not directly interfere with
subsequent reactions. In fact, a little PEG usually is benificial
due to its molecular crowding effect causing concentration of the
substrates. However, people often discover that PEG precipitants
appear badly inhibited for further reaction. This is because of the
other non-specific crud that was co precipitated with the DNA.
PEG itself can be easily removed with chloroform extraction; but, of
course, getting rid of the crud will depend on exactly what the crud
is.

http://www.bio.net/mm/methods/1995-May/028884.html

--------------------------------------------------
Note added at 5 days (2013-09-30 14:44:47 GMT)
--------------------------------------------------

*should read "suspended in a PEG polymer lattice BEFORE FILTRATION".

--------------------------------------------------
Note added at 6 days (2013-10-01 12:49:33 GMT) Post-grading
--------------------------------------------------

Thank you very much Sandra, my explanation certainly wasn't the most lucid, because I kept on finding these different uses of PEG in purification, filtration, inactivation, etc....but I'm very glad that you read the info. I encountered. Together they help to fill in the pieces of the puzzle.
Something went wrong...
3 KudoZ points awarded for this answer. Comment: "Hi Neil, I have read all your explanations! ther were very helpful. Thank you!"
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